Information about HPU testing

The HPU-Test® is, like the PKU-Test, a screening test. The PKU-Test is better known as the heel prick in babies. It is tested which babies can later develop the disease (PKU, phenylketonuria).

The HPU-Test® is not performed in the blood, but in the urine. Here too, it is looked at who can get symptoms later by HPU.

HPU does not develop into a specific disease, but to a range of symptoms, from mild to very severe, such as joint complaints (hypermobility or over-mobility), menstrual complaints, reduced fertility, pelvic instability, depression, cardiovascular disease, anemia, diabetes, thyroid problems and excess weight after pregnancy. HPU is mainly found in women.

If you recognize yourself (in part) in the complaint pattern that can be caused by HPU, you may ask yourself the question: “Do I have HPU?”

Especially when you recognize the symptoms that can arise from direct vitamin B6, zinc and manganese deficiencies, it may be useful to find an answer. In particular, one of the direct symptoms is typical of people with HPU: an increased mobility of the joints (hypermobility). For example, many women can put their little finger straight back, or cross their thumb (see photo1 and photo 2). Also over-mobility of the temporomandibular joint is common.

In addition, many women are tired with HPU. Fatigue in HPU can be due to hypoglycaemia, reduced liver capacity, low histamine levels and / or adrenal activity.

Countless women with fatigue problems walk the medical and alternative circuit in search of a cure.

Tired patients are often treated incorrectly or incorrectly diagnosed. An example is the ‘residual diagnosis’ chronic fatigue syndrome (CFS or ME). Some of the women who live with the label ‘ME’ or ‘CFS’ actually have HPU and can therefore be treated.

If you want an answer to the question whether you have HPU, you can print out the questionnaire that you find on this site. The questionnaire is also included in the book Do you have HPU?

HPU test posibilities

The HPU study consists of a number of test options. In addition to the HPU questionnaire (see elsewhere on this website), the simplest option is to test the urine for the presence of the hemopyrrollactam complex. This is a simple urine determination for which a small amount of mid-flow morning urine is required. The test costs € 57,23. Persons who are chronically ill or bedridden should consider an HPU test based on a 24-hour urine. The costs are € 69,10.

HPU screening

The HPU screening consists of multiple tests that are carried out with the help of a blood sample. The HPU screening consists of a fructosamine assay, an IgA total gluten test, a whole blood histamine assay and a simple hormone test. The costs of this blood test are € 151.50 (€ 164, – incl. Basic rate).

There is an even more extensive possibility, namely:

HPU screening with extensive allergy research

This research does not only include fructosamine, whole blood histamine, IgA total gluten, IgE total, but also a complete allergy study on 100 foods and fungi. This test is recommended for intestinal complaints, allergic reactions such as hay fever, eczema, and the like. The costs of this extensive blood test are € 317,75. (€ 330,25 including base rate). The questionnaire also contains an advice about any further tests that may be carried out.

False positive results

Hemopyrrolactam complex (HPL) is excreted in the urine in most porphyria diseases. It may be that there is another porphyria disease. The concentration of HPL can be influenced by chemical load, but also by food. The highest concentration of HPL in the urine is usually found a few hours after the hot meal. Alcoholism, hyperthyroidism (increased thyroid processing) and mononucleosis (infectious disease with lymph node swelling) can influence the outcome.

The following diseases and conditions may increase the rash: pernicious anemia, Bartter’s syndrome, liver cirrhosis, Crigler-Najjar’s disease, Gilbert’s disease, hepatitis, spherocytosis, malaria, sickle cell disease, shortly after a heart attack, physical stress, psychological stress and shortly after operation or accident. In this case, there is not necessarily a false-positive result.

Fals negative results

Your result may be false negative if you have regularly used vitamins, in particular B vitamins and / or zinc / manganese, for example in the form of multivitamins / minerals. The amount of HPU excreted gradually decreases and in the short period that you have not used any supplements just before the test will increase again, but perhaps not enough to get a positive result.

Overall, you can assume that if you use more than 100 mg of vitamin B6 over a longer period of time (in a period of low stress) you may expect a negative result. The result is also reduced if you had to urinate several times the night before or if you use diuretics (water tablets).

Very specific drugs can negatively affect the amount of HPL secreted. Anemia caused by other causes than HPU, such as a deficiency of vitamin B12, sickle cell anemia and reduced bilirubin levels may also cause lower concentrations of HPL in the urine.